| Applications: |
ELISA |
| Reactivity: |
Porcine |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This ACTH Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in porcine cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
1.5h |
| Detection: |
Colormetric |
| Specificity: |
This kit recognizes Porcine ACTH in samples. No significant crossreactivity or interference between Porcine ACTH and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Specificity | This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
Sample Type Range (%) Average Recovery (%)
| Serum(n=8) | 90-104 | 96 |
| EDTA plasma(n=8) | 92-106 | 99 |
| Cell culture media(n=8) | 91-103 | 96 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.87 | 137.14 | 399.81 | 52.45 | 128.28 | 373.56 |
| Standard deviation | 2.66 | 5.69 | 21.55 | 3.66 | 7.13 | 18.53 |
| CV (%) | 5.23 | 4.15 | 5.39 | 6.98 | 5.56 | 4.96 |
12 months for
antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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